Emirates Journal of Food and Agriculture 35(12): 1-11, doi: 10.9755/ejfa.2023.3204
Polyphenol galangin induces the ROS and ER stress-mediated intrinsic and extrinsic apoptotic pathways towards human colon carcinoma HCT-116 cells
expand article infoBo Wang‡, Xin Fu§, Li-Li Liu§, Fei-Fei Wu|, Xin-Huai Zhao
‡ State Key Laboratory of Palaeobiology and Stratigraphy, Nanjing Institute of Geology and Palaeontology, Chinese Academy of Sciences, No.39, East Beijing Road, Nanjing 210008, China§ College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150040, China, China| School of Biological and Food Engineering, Guangdong University of Petrochemical Technology, Maoming 525000, China, China¶ School of Biological and Food Engineering, Guangdong University of Petrochemical Technology, Maoming 525000, China;Research Centre of Food Nutrition and Human Healthcare, Guangdong University of Petrochemical Technology, Maoming 525000, China., China
© Bo Wang, Xin Fu, Li-Li Liu, Fei-Fei Wu, Xin-Huai Zhao.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation: Wang B, Fu X, Liu L-L, Wu F-F, Zhao X-H (2023) Polyphenol galangin induces the ROS and ER stress-mediated intrinsic and extrinsic apoptotic pathways towards human colon carcinoma HCT-116 cells. Emirates Journal of Food and Agriculture 35(12): 1-11. https://doi.org/10.9755/ejfa.2023.3204
Open Access
Abstract

A natural anti-oxidant galangin, a flavonoid compound rich in the root of galangal, has been proved to exert anti-proliferation and apoptosis induction towards various cancer cells. However, its activities and molecular mechanism against colorectal cancer cell lines (e.g. HCT-116) is still unclear. The aim of this study was to reveal the inhibition and apoptosis induction of galangin in the HCT-116 cells. The results of CCK-8 assay demonstrated that galangin at 20-160 μmol/L inhibited the HCT-116 cells growth in a dose-time-dependent manner. Galangin changed cell morphology, arrested cell cycle at G0/G1 phase, decreased mitochondrial membrane potential, increased intracellular reactive oxygen species (ROS) and Ca2+ and induced early apoptosis in the HCT-116 cells. Based on western blotting results, on one hand, galangin at 80-160 μmol/L up-regulated pro-apoptotic proteins expression such as AIF, PIG3, and Bax, and induced mitochondrial pathway by up-regulating the levels of cleaved caspase-8, cleaved caspase-7, cleaved caspase-9 and cleaved caspase-3, as well as cleavage of poly (ADP-ribose) polymerase (PARP). On the other hand, galangin also induced endoplasmic reticulum (ER) stress in the cell via up-regulation of CHOP and DR5 and then activation of caspase cascades. The results illustrated that galangin induced apoptosis towards the HCT-116 cells through the ROS- and ER stress-mediated both intrinsic and extrinsic apoptotic pathways.

KEYWORDS: galangin; human colon carcinoma cells; apoptotic mechanism; reactive oxygen species; endoplasmic reticulum stress

 

Keywords
galangin; human colon carcinoma cells; apoptotic mechanism; reactive oxygen species; endoplasmic reticulum stress